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Fig. 5 Foxe1 mutants and wild types are severely affected by 1% ethanol exposure. (A) Representative images of the effects of 1% ethanol exposure on bone and cartilage development in wild type and mutant larvae at 120 hpf. At 1% ethanol concentration, most of the fish were mildly phenotypic, mostly showing defects in bone development. In 17% (6/35) of wild types and 28% (14/49) of mutants more severe phenotypes were observed. In case of a more severe phenotype, the exposed wild types retained more cartilage elements than the exposed mutants. Cb: Ceratobranchial, ch: Ceratohyal, et: Ethmoid plate hy: Hyosympletic, m: Meckels, o: Otoliths, p: Parachordal, pa: Palatoquadrate. (B) Unexposed control individuals had normal ethmoid plates, while ethanol-exposed larvae had hypoplastic ethmoid plates with dents in the anterior edge or lack of cells in the midline. The incidence was higher in mutants 45/102 (44%) than in wild types 21/92 (22%). Scale bar (A) 200 μm, (B) 50 μm. Data were analyzed using Chi-square test.