ZFIN Image: Tan et al., 2025, Figure 5

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Figure 5

Overexpression of a dominant-negative form of Elf1 (DN-Elf1) in macrophages disrupts their response to injury. (A) Time-lapse imaging of macrophage infiltration at 3 dpf in control zebrafish and zebrafish expressing DN-Elf1 specifically in macrophages. The background transgenic line used was Tg(mpeg1:Gal4FF/UAS:eGFPcaax). Imaging of the caudal fin region was initiated two hours post-amputation. The white dashed line marks the position of transection. Scale bar: 50 μm. (B) Quantification of macrophage numbers within the damage shown in (B). Data were analyzed using two-way ANOVA followed by Sidak’s tests, with n ≥ 7, and shown as mean ± S.E.M. Statistical significance indicated as ** p < 0.01, *** p < 0.001, and **** p < 0.0001.

Acknowledgments

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