Morpholino knockdown-mediated screening of Ets family members in zebrafish macrophage development. (A) Representative confocal images showing macrophages in the head region of Tg(mpeg1:eGFP) zebrafish larvae at 3 dpf following morpholino-mediated knockdown of Ets family members. (B) Quantification of macrophage numbers in the head regions of the transgenic zebrafish shown in (A). Data were analyzed using one-way ANOVA followed by Dunnett’s test, with n ≥ 20, and shown as mean ± S.E.M. (C) Representative confocal images showing macrophages in caudal hematopoietic tissue (CHT) at 3 dpf following morpholino-mediated knockdown of Ets family members. (D) Quantification of macrophage numbers in the CHT of the transgenic zebrafish shown in (C). Data were analyzed using one-way ANOVA followed by Dunnett’s test, with n ≥ 15, and shown as mean ± S.E.M. Statistical significance is indicated as ns (p ≥ 0.05), **** p < 0.0001. Scale bar: 50 μm.
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