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Fig. 2 The Cerebral blood vessel network was reconstructed in approximately a week. (A) Temporal imaging of blood vessels and blood flow pre- and post-photothrombosis induction. The white dotted lines demarcate the laser irradiation area in the zebrafish IS model telencephalon. The magenta-dotted lined area corresponds to Fig. 2C. White arrowheads indicate the shrinking of the vessel. Cyan arrowheads indicate the newly formed vessel. A yellow arrowhead indicates spontaneous bleeding. Scale bar, 500μm. (B) Temporal alteration of BFR in control and IS model fish telencephalon. Each gray bar represents the average of BFR. Each dot represents an individual value. ** and **** denote significant differences. **, p < 0.01; ****, p < 0.0001, Kruskal-Wallis with Dunn’s multiple comparison test. n = 4 (WT), 10 (IS model). (C) Comparison of the blood vessel pattern between pre-laser-irradiation and 14 dpi in the control. A yellow panel represents the vessel pattern pre-irradiation, and a cyan panel represents that at 14 dpi. (D) Comparison of the blood vessel pattern among pre-laser-irradiation, 3dpi, and 14dpi in IS model telencephalon. A yellow panel represents the vessel pattern pre-irradiation, a magenta panel represents that at 3 dpi, and a cyan panel represents that at 14 dpi. Lower panels show the merged view of pre- and 3 dpi, 3 dpi and 14 dpi, and pre- and 14 dpi, respectively. Cyan arrowheads indicate vessels that have disappeared, white arrowheads indicate newly formed vessels, and green arrowheads indicate vessels that were newly formed at 3 dpi but pruned by 14 dpi.