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Fig. 1 Telencephalon-specific photothrombosis induction in adult zebrafish. (A) Schematic representation of photothrombosis induction in the adult zebrafish brain. The anesthetized fish was positioned dorsally and immobilized with a sponge. Rose bengal was administered intraperitoneally. Subsequently, the fish was repositioned ventrally and immobilized with a sponge, and a green laser was irradiated under confocal microscopy. A peristaltic pump was employed to supply anesthetic to the fish’s mouth. The illustration was created with BioRender.com. (B) The amalgamation of rose bengal injection and laser irradiation could induce thrombosis. White dotted lined areas demarcate the laser irradiation area. Scale bar, 500μm.