Fig. 6

Fig. 6

The Cholesterol Biosynthesis pathway is coordinately regulated in the retinal GCL by srebf2 knockdown or constitutively active overexpression at 3 dpi as measured by LCM-seq. A) The six experimental groups being compared by LCM-seq and the heat shock treatment regimen. N = 4 fish per treatment group, two males and two females. B) The mev/chol pathway is up regulated 3 dpi, down regulated by srebf2-MO2 treatment, and up regulated by transgenic (Tg) constitutively active srebf2 over expression in control MO and srebf2-MO2 treated groups (FDR < 0.25). GSEA based on zebrafish Wikipathway. C) Venn diagram for differentially expressed genes (|FoldChange| ≥ 1.5) in the different comparison groups identifies 464 common genes. D) WikiPathways over-representation analysis of the 464 common genes in C enriches for the mev/chol pathway. E) Heatmap of mev/chol synthesis gene expression in each experimental group. 3dpi, uninjured, C-MO, and srebf2-MO2 groups used wt zebrafish; Tg C-MO + HS and Tg srebf2-MO2 + HS used Tg(hsp70l: nSrebf2-2AmCherry, cmlc: GFP) zebrafish with daily heat shock (HS)