FIGURE 4

FIGURE 4

Gdf11 forms heterodimer with Spaw and regulates DFC specification through TGF‐β signal. (A) Immunoprecipitation between zGdf11‐Flag and zSpaw‐Myc in HEK 293T cells. (B) Western blot results of overexpressed zGdf11‐Flag and zSpaw‐Myc in HEK 293T cells with or without SB431542 treatment. (C) foxj1a and its target gene expression using WISH in WT or gdf11 mutants with or without treatment. (D–G) Expression levels of foxj1a and its target genes. Error bars represent the standard deviation. Statistical significance was determined using a two‐tailed unpaired t‐test. (H) Visualization of KV cilia at the 10‐somite stage using an anti‐acetylated tubulin antibody in both WT or gdf11^−/− mutants with or without SB431542/SRI‐011381 treatment. Scale bar, 50 μm. (I, J) Number (I) and length (J) of KV cilia in WT and mutant embryos with or without SB431542/SRI‐011381 treatment. Graph indicates the mean of cilia number and length, error bars represent the standard deviation.