FIGURE 2

FIGURE 2

gdf11 is essential for left–right patterning. (A) Cardiac looping and liver/pancreas positions analysed at 48 hpf. Heart looping visualized by in situ hybridization with cmlc2 probe at 48 hpf (top panel). A, atrium; V, ventricle. Liver/pancreas positions visualized by in situ hybridization with hhex probe at 48 hpf (bottom panel). L, liver; P, pancreas. (B) Graph showing the percentage of embryos with normal D‐looping (dark blue), no looping (yellow), or L‐looping (green). (C) Graph showing the percentage of embryos with normal liver/pancreas position (dark blue), bilateral liver/pancreas position (yellow), or reverse liver/pancreas position (green). (D) spaw (upper panel) and pitx2 (lower panel) expression in WT and gdf11^−/− mutants at 21 or 22 ss. (E, F) Percentage of spaw (E) and pitx2 (F) expression in WT and gdf11^−/− mutants. Normal (dark blue), reverse (yellow), or absent (green). (G, H) Graphs depicting the expression of midline ntl at 19 ss (G) and the percentage of ntl expression in WT and gdf11^−/− mutants (H). (I, J) Graphs depicting the expression of lft1 at 16 ss (I) and the percentage of lft1 expression in WT and gdf11^−/− mutants (J).