FIGURE 1

FIGURE 1

gdf11 expression pattern and mutant construction. (A) Whole‐mount in situ hybridization with gdf11 from the 2‐cell stage to 72 hpf. gdf11 sense probe was utilized as negative control at 1 cell stage. (B) Schematic picture showing the wild type and mutated site of gdf11^−/− mutants. (C) Western blots of lysates from WT and gdf11^−/− embryos using antibodies against Gdf11 with β‐tubulin as a loading control. (D) Alcian blue staining in both WT and gdf11 mutants. gdf11 mutants exhibit defects in the alignment of upper and jaw elements, the angle of ch. articulation, and the morphology of the op. The defects of the alignment of upper/jaw elements and the morphology of the op in gdf11 mutant were marked with straight lines and arrows in the picture. op，opercle; ch，ceratohyal. Scale bar, 50 μm. (E) Live images of WT and gdf11^−/− mutants from 3 to 5 dpf. Black triangle indicates craniofacial cartilage. Scale bar, 100 μm.