Fig. 5
Knockdown of Ndufb7 reduces the neuronal volume of the brain.
Transgenic Tg(Huc:kaede) zebrafish embryos were treated as described in Fig. 3, cultured until 48 hours post-fertilization, observed using a 10X objective, and photographed under a black field using a GFP cube under confocal microscopy. A All images are presented in lateral view. B The midbrain (mb) and hindbrain (hb) are pseudocolored in yellow and white, subjected to volume measurement using the Metamorph software, as shown in C midbrain and D hindbrain. The volume of each brain compartment was measured in voxels. Data from three independent experiments are presented, analyzed, and shown as described in Fig. 3. NS not significant; **p < 0.01; ***p < 0.001.
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