Fig 1

Fig 1

SVCV infection causes hypoxia-induced symptoms and promotes hypoxia-inducible gene expression in zebrafish. (A-C) SVCV infection caused symptoms in zebrafish, similar to those observed under hypoxic conditions. After 24 h of starvation, adult zebrafish (3 mpf) were injected intraperitoneally with SVCV (5 × 10⁷ TCID₅₀/mL, 10 µL/individual). Twenty-four hours later, infected zebrafish were observed swimming at the surface of the water with their opercula erect and breathing rapidly. Based on the video recordings, we counted the number of times the gill covers opened and closed in three groups of zebrafish. (D) qPCR analysis of SVCV-P, SVCV-G, SVCV-N, SVCV-M, and SVCV-L mRNA in the brain of zebrafish (3 mpf) with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL, 10 µL/individual). (E) qPCR analysis of ifn1 and lta mRNA in the brain of zebrafish (3 mpf) with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL, 10 µL/individual). (F) qPCR analysis of vegfaa and phd3 mRNA in the brain of zebrafish (3 mpf) with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL, 10 µL/individual). (G) qPCR analysis of SVCV-P, SVCV-G, SVCV-N, SVCV-M, and SVCV-L mRNA in the spleen of zebrafish (3 mpf) with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL, 10 µL/individual). (H) qPCR analysis of ifn1 and lta mRNA in the spleen of zebrafish (3 mpf) with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL, 10 µL/individual). (I) qPCR analysis of vegfaa and phd3 mRNA in the spleen of zebrafish (3 mpf) with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL, 10 µL/individual). (J) Representative images of Tg (HRE-sv40mp : GFP) zebrafish larvae (3 dpf) infected with or without infection with SVCV (5 × 10⁷ TCID₅₀/mL). (K) Quantitation of total intensity in (J).