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Fig. 4 AURKBi and IR display comparable tumor suppressive activity in vitro and ex vivo. A) Experimental procedure for the in vitro cell viability assays shown in B and C. B) Viability of HD-MB03 and C) D425 cells in response to IR (1,8 Gy) and Barasertib (20 nM) treatment alone or in combination after 48 h (n=3 biological replicas). D) Confocal microscopy IFA of OCSCs 6 days after treatments with Barasertib 100 nM and IR 1.8 Gy. Green: Anti-human nucleoli antibody, red: Click-iT® EdU, blue: anti-Cabindin Click-iT® EdU, yellow: anti-GFAP. Human nuclei are displayed in inverted grey scale and EdU incorporation as volume renderings from Z-stack images. E-H) Box plots of TV and rPV of OCSCs from D. (n≥3 biological replicas).