ZFIN Image: Guo et al., 2025, Figure 4.

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Figure 4.

Conditional Cre/Lox RNAi genetic system. (A) Schematic representation of the driver transgene, with here a ubiquitous promoter, named ubi:iCre. Tissue-specific promoters can obviously be used. (B) Schematic representation of the silent/conditional gene-silencing responder transgene. In the absence of iCRE, only BFP will be expressed and the animal will remain unaffected by the RNAi cassette, thereby could be maintained without special care. In the presence of iCre, genetic recombination will lead to excision of the BFP cassette and expression of mKate along with the miRNAs of choice; with here 4× anti-smn1 miRNAs, resulting in potent zebrafish smn1 knockdown. This recombination is irreversible.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.