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Fig. 4 ΔCt values and fold-change in gene expression of preproinsulin essential in both adult zebrafish models of diabetes. qPCR analysis of zebrafish preproinsulin (ppins) (A) in control vs. the 2% glucose-induced nonobesity model and the DIO model. ΔCt values were taken as a mean of the population (n = 6 and n = 7 for control and experimental groups, respectively), and a fold-change was determined for each candidate gene (B). Data were analyzed using one-way analysis of variance (ANOVA); β-actin is the reference gene; values are mean ± SD vs. control; **p < 0.01.
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