Fig. 1 Experimental approach to establishing type 2 diabetes mellitus (T2DM) zebrafish models. Adult zebrafish [4–6 months postfertilization (pf), males and females] were (A) continuously exposed to a 0% (control group) or 2% (experimental group) glucose solution for 40 days or (B) fed 68 calories (control group) or 408 calories (experimental group) of Otohime per fish per day for 10 weeks. Bodyweight, fasting blood glucose measurements, and RNA isolation were performed on all carcasses following euthanasia. mRNA was transcribed to cDNA and used for quantitative polymerase chain reaction (qPCR) analysis.
Image
Figure Caption
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Zebrafish