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Fig. 5 Treatment with topical saline improves burn wound healing potential of cultured human skin. (A) Experimental timeline for burn wounding of cultured human skin. The indicated treatments were applied topically for 1 day post-burn (dpb), and frozen skin sections were taken at each time point indicated. (B) Representative images of frozen skin sections stained with LDH to assess tissue viability. (C) Image showing method of quantification for burn depth. Blinded analysis was performed to measure LDH stained tissue. (D) Quantification of burn depth over time with the indicated treatment. N = 7–9 replicates from 3 subjects. Each subject contained up to 3 technical replicates. Asterisk indicates p < 0.05 by two-way ANOVA.