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Fig. 4 Rotenone exposure accompanying neuroinflammation in developing zebrafish embryos. Wild-type embryos aged 7–8 h post fertilization (hpf) were exposed with 30 nM rotenone to 72 hpf. Tg(mpeg1:EGFP) embryos were exposed to rotenone at 72 hpf for 0.5, 1, 2, 4, 8 and 12 h. The mRNA expressions of (A) cd68 (cluster of differentiation 68), (B) csf1r (colony-stimulating factor 3 receptor), (C) apoeb (apolipoprotein E) (D) aif1 (allograft inflammatory factor 1), N = 20. (E) Midbrain images using confocal microscopy gated with red dots, Scale bar: 10 µm, N = 6. (F) Quantification of mpeg1-expressing number of cell and (G) representative images of Tg(mpeg1:EGFP) at 0.5, 1,2,4,8, and 12 h post exposure (hpe) of rotenone, Scale bar: 10 µm, N = 4–12. Data presented as the mean ± SD. SD: standard deviation. * p < 0.05, * * p < 0.01, * ** p < 0.001, ns: not significant.
Reprinted from Journal of hazardous materials, 480, Ranasinghe, T., Seo, Y., Park, H.C., Choe, S.K., Cha, S.H., Rotenone exposure causes features of Parkinson`s disease pathology linked with muscle atrophy in developing zebrafish embryo, 136215136215, Copyright (2024) with permission from Elsevier. Full text @ J. Hazard. Mater.