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Fig. 3 Neutrophil responses to SVCV infection are impaired in the absence of intestinal microbiome. Neutrophils (A) and macrophages (C) were imaged in mock or SVCV-infected GF or conventionalized transgenic zebrafish at 48 hpi. Scale bar, 500 μm. The number of neutrophils (B) and macrophages (D) were analyzed (n = 3). Effect of myeloid cell depletion (Spi1b MO) (E) or selective depletion of neutrophils (Csf3r MO) or macrophages (Irf8 MO) (F) on viral replication in GF or conventionalized zebrafish at 48 hpi (n = 4, pool of 30 zebrafish larvae per sample). G Effect of neutrophils depletion (Csf3r MO) on virus titer in GF or conventionalized zebrafish at 48 hpi (n = 4, pool of 30 zebrafish larvae per sample). H Effect of myeloid cell depletion (Spi1b MO) or selective depletion of neutrophils (Csf3r MO) on IFNΦ1 expression in GF or conventionalized zebrafish at 48 hpi (n = 4, pool of 30 zebrafish larvae per sample). B, D, E–H Unpaired t-test. *p < 0.05, **p < 0.01, n.s., not significant