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Fig 4 Measuring gut motility using kymography.
(A,C,E) Representative bright-field images showing the (A) anterior, (C) mid and (E) posterior regions of the intestine of SD controls larvae at 7 dpf, and (B,D,F) their respective kymographs. Green and red arrows (in A, C, and E) and lines (in B, D, and F) indicate anterograde and retrograde contractile activity, respectively, and the yellow rectangle (in F) indicates the rectal waves. Scale bars are 50 μm (A,C,E) and 40 μm over 50 s (B,D,F).
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