FIGURE 4

FIGURE 4

Depletion of insulin production elevates glucose levels, but not ROS, in ins:epNTR-mCherry zebrafish. (A) Quantification of the glucose levels (mg/dL) in Ins:epNTR-mCherry larvae treated with DMSO and MTZ at 9 dpf, ****p < 0.0001, t-test (n = 20 larvae per one data point, total n = 100 larvae per group). (B) Quantification of the ROS intensity in the PLLg cells of Ins:epNTR-mCherry expressing Tg (tubb2b:dsred) larvae presented in panels (D’) and (E’) (n = 10 larvae per group). (C) Quantification of the number of PLLg cells of the transgenic larvae presented in panels (D”) and (E”) (n = 11 larvae per group). (D–E”)In-vivo fluorescence images showing PLLg cell and CellROX ROS detector in the transgenic larvae treated with DMSO (D–D”) or MTZ (E–E”) at 9 dpf. Scale bar, 20 μm in (D).