ZFIN Image: Lee et al., 2024, FIGURE 2

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Figure Caption

FIGURE 2

Depletion of insulin production caused peripheral neuropathy in the peripheral lateral line nerve of ins:epNTR-mCherry zebrafish. (A–C)In-vivo fluorescence images showing sensory nerve endings in Ins:epNTR-mCherry expressing Tg (tubb2b:dsred) larvae treated with DMSO (A) and MTZ (B) at 9 dpf. In-vivo fluorescence images showing sensory nerve endings in Tg (tubb2b:dsred) siblings treated with MTZ (C) at 9 dpf. (D) Quantification of the number of axon bifurcation from panel (A–C), ****p < 0.0001, one-way ANOVA (n = 12 larvae per group). (E–F’) Fluorescence images showing sensory nerve endings with postsynaptic MAGUK⁺ puncta in Ins:epNTR-mCherry expressing Tg (tubb2b:dsred) larvae treated with DMSO (E,E’) and MTZ (F,F’) at 9 dpf. (G) Quantification of fluorescence intensity of MAGUK⁺ puncta in the transgenic larvae presented in panels (E’) and (F’), ****p < 0.0001, t-test (n = 12 in DMSO group and n = 10 in MTZ group). Scale bar, 10 μm in (A) and (E).

Acknowledgments

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