|
Fig. 3 Autophagy promotes cell survival independently of the proteasome activity (A) LysoIP performed after 3 h of 50 nM CPT (nM) or 1 μM CPT (μM) and the proteasome inhibitor bortezomib (BTZ) (n = 3). (B) Clonogenic assays performed simultaneously with low- or high-dose CPT (n = 3). Error bar, SEM. (C) LysoIP in S-phase HeLa cells after 3 h of 50 nM CPT and 1 μM ATR inhibitor VE-822 (ATRi) or 1 μM ATM inhibitor KU-55933 (ATMi) (n = 3). (D) Co-immunoprecipitation of p97-Strep in S-phase HEK293T cells after 1 h of 50 nM CPT (nM) or 1 μM CPT (μM) or 1 μM ATR inhibitor VE-822 (ATRi) (n = 2). (E) Immunofluorescence of protein aggregates using the Proteostat dye after 24 h of 50 nM CPT and quantification (n = 3). Scale bar, 10 μm. Error bar, SD. Two-way ANOVA. ∗∗p < 0.005; ∗∗∗p < 0.0005; ns, not significant. See also Figure S3.
Reprinted from Cell, 187(20), Lascaux, P., Hoslett, G., Tribble, S., Trugenberger, C., Antičević, I., Otten, C., Torrecilla, I., Koukouravas, S., Zhao, Y., Yang, H., Aljarbou, F., Ruggiano, A., Song, W., Peron, C., Deangeli, G., Domingo, E., Bancroft, J., Carrique, L., Johnson, E., Vendrell, I., Fischer, R., Ng, A.W.T., Ngeow, J., D'Angiolella, V., Raimundo, N., Maughan, T., Popović, M., Milošević, I., Ramadan, K., TEX264 drives selective autophagy of DNA lesions to promote DNA repair and cell survival, 5698-5718.e26, Copyright (2024) with permission from Elsevier. Full text @ Cell