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Fig. 5 Phosphorylated extracellularly regulated kinase (p-ERK) assay confirmation of a kisspeptin receptor (KISS1R) agonist. A, The Venn diagram shows the p-ERK assay confirmation rate of KISS1R agonist candidates in the HEK293-KISS1R cells. B, Dose-response curves demonstrate that musk ambrette treatment stimulated ERK phosphorylation in HEK293-KISS1R cells but not in wild-type (WT) cells. All values represent the mean ± SD (n = 3 replicates).
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