ZFIN Image: Huysseune et al., 2023, Figure 1

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Figure Caption

Figure 1

Live staining of early postembryonic zebrafish embryos with DAF-FM DA. (A,B). Live staining with DAF-FM DA, according to published protocols, reveals signals in bony structures (opercular, arrow; cleithrum, arrowhead), as well as in the heart (asterisk) and around the notochord, which is consistent with earlier reports. Axio Observer image. (C–E). Three µm GMA sections of the specimen shown in (A,B), counterstained with DAPI, showing the opercular bone (C), notochord (D), and bulbus arteriosus (E). Note that nuclei lie outside fluorescent domains. Scale bars in (A,B) = 200 µm, in (C,E) = 20 µm, in (D) = 10 µm.

Acknowledgments

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