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Fig. 5 Long-term expression of pS/MAR-USH2A in the zebrafish retina GFP expression was observed in the retina of WT and ush2au507 zebrafish injected with pS/MAR-CMV-USH2A at the one-cell stage. Anti-turboGFP was used to aid GFP detection on retinal sections at ages 6 days post-fertilization (dpf) (A–C), 1 month post-fertilization (mpf) (D–F), 2 mpf (G–I), 3 mpf (J–L), 6 mpf (M–O), and 12 mpf (P–R). Sections were counterstained with DAPI nucleic acid stain (blue). OS, photoreceptor outer segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; ON, optic nerve. Scale bars, 25 μm.