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Fig. 4 pS/MAR-USH2A vector expression in zebrafish (A) WT and ush2au507 zebrafish were micro-injected with pS/MAR-CMV-USH2A at the one-cell stage. GFP expression was detected in injected WT and ush2au507 larvae at 5 dpf. (B) RT-PCR analysis detected human USH2A (hUSH2A) expression in injected (inj) zebrafish and not in un-injected (un-inj) larvae. ef1a gene expression was used as a control. No reverse transcription (no RT) and water (H2O) controls were performed. The full gel is available in the supplemental information.
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Full text @ Mol. Ther.