Figure 5.

Figure 5.

DPC analysis in Tdp1 and Sprtn deficient embryos under physiological conditions and after CPT (10 µM, 1 h) and FA (5 mM, 30 min) treatment. (a) DPCs were isolated from 2 dpf embryos using the RADAR assay (30 embryos per condition, n = 4), resolved on the SDS acrylamide gel, and stained with silver (left panel-low exposure; right panel-high exposure). Dot blots showing DNA loading controls for DPC analysis prior to benzonase treatment are shown below (DPC equivalent of 200 ng of total DNA was loaded per well). (b) Quantification of (a). Quantifications of LMW DPCs (protein size less than 40 kDa) (c), MMW DPCs (40–150 kDa) (d) and HMW (greater than 150 kDa) (e) from (a). Data represent mean fold change to WT ± s.d. (n = 4). Statistical significance was established using an unpaired Student's t-test (*p < 0.05, **p < 0.01, ***p < 0.001 and ^#p < 0.0001).