IMAGE

Figure 3.

ID
ZDB-IMAGE-231005-14
Source
Figures for Anticevic et al., 2023
Image
Figure Caption

Figure 3.

H3-DPC levels are increased in vivo in tdp1 mutant fish line and in RPE1 cells with TDP1 deficiency. (a) Western blot showing H3-DPC levels in tdp1 mutant embryos in combination with sprtn knockdown and CPT (10 µM, 1 h) treatment. Total DPCs were isolated from 2-day-old embryos, separated by SDS–PAGE (DPC equivalent of 200 ng DNA per well) and detected with H3-specific antibody. (b) Quantifications of H3-DPCs from four biological replicates with mean (± s.d.) fold change to endogenous H3-DPCs in WT embryos. (c) Western blot analysis of H3-DPCs in zebrafish embryos and (d) corresponding quantification after FA treatment (5 mM for 30 min) (n = 4). (e) Dot blots showing H3-DPCs after silencing TDP1 and/or SPRTN before and after CPT exposure in RPE1 cells (50 nM CPT, 1 h) and DNA loading controls. Equivalent of 200 ng DNA of total DPCs was loaded per sample. (f) Quantification of H3-DPC analysis in RPE1 cells (n = 3). (g) Dot blots showing H3-DPCs after silencing TDP1 and/or SPRTN before and after FA exposure (1 mM FA, 20 min) in RPE1 cells and DNA loading controls and (h) corresponding quantification (n = 3). Results are presented as mean ± s.d. with statistically significant changes, determined using an unpaired Student's t-test, indicated with *p < 0.05, **p < 0.01, ***p < 0.001 and #p < 0.0001.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Open Biol.