Fig. 8

Structural alignment of tyrosinases (A,B) and predicted glabridin–tyrosinase complex (C,D, closed up). (A,B) represent the structural alignment of three tyrosinases from mushroom (gray), murine (brown), and zebrafish (blue) in cartoon and surface formats. The mavConservation score is used (in A,B) to determine the degree of conservation among tyrosinases. For example, red represents a value of 1, indicating high conservation (active site). Blue represents a value of 0.333, indicating low conservation. White represents a value of 0.667, indicating medium conservation. (C,D) exhibit a predicted enzyme-inhibitor complex between glabridin and tyrosinases. Gray indicates a glabridin–mushroom tyrosinase. In comparison, brown represents a complex between glabridin–murine’s tyrosinase. Finally, blue exhibits a complex from zebrafish’s tyrosinase and glabridin. Tropolone (Black, a native competitive tyrosinase inhibitor) indicates a catalytic site on aligned tyrosinases [20].