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Butko et al., 2015 - Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo

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Fig. S1

gata2a is expressed within the vasculature, and is required for vascular integrity. (A) In situ hybridization of gata2a expression in the PLM at the 8-somite stage (13 hpf) (B) Expression of gata2a at 25 hpf. (C) Confocal microscopy shows a lateral view of the trunk region of gata2a:GFP;kdrl:mCherry^mem at 25 hpf. (C′) Digitally reconstructed transverse view of the trunk region in (C). (D-E) Pooling of red blood cells observed by o-dianisidine staining in 2 dpf siblings (D) and gata2a^um27 mutants (E). (F) Confocal microscopy of the head vasculature and erythrocytes in a 2 dpf lmo2:GFP;gata1a:DsRed;gata2a^um27-/- embryo. White arrows denote extravascular erythrocytes.

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