Fig. 4

In vitro and in vivo evaluations of the antiviral activity of Igldcp. A) qPCR detection of SVCV, IPNV and RGNNV in the ZF4, RTG-2 and SSN-1 cell lines, respectively, transfected with the expression plasmid pcDNA3.1-igldcp or the corresponding empty control plasmid. The expression levels of the target viral genes were normalized to the expression of the 18S rRNA gene corresponding to the origin of the cell line. B) Effect of the overexpression of igldcp on zebrafish larvae. Zebrafish embryos were microinjected with the plasmid pcDNA3.1-igldcp or pcDNA3.1, and at 3 dpf, the larvae were infected or not infected with RGNNV. The expression of igldcp in the different groups was evaluated by qPCR at 24 hpi and normalized to the expression of the 18S rRNA gene. The survival rate after the challenge was determined over a period of 11 days and is presented in Kaplan‒Meier survival curves. C) Effect of igldcp knockdown on zebrafish larvae. Zebrafish embryos were microinjected with Mo-igldcp or Mo-C, and at 3 dpf, the larvae were infected or not infected with RGNNV. The replication of RGNNV was determined 24 hpi by qPCR detection of the capsid protein (CP)-encoding gene, which was normalized to the expression of the zebrafish 18S rRNA gene. The survival rate after the challenge was determined over a period of 11 days and is presented in Kaplan‒Meier survival curves. The qPCR data are presented as the means ± SEMs of 3–5 independent biological replicates. Statistically significant differences are displayed as follows: ∗∗∗ (0.0001 < p < 0.001), ∗∗ (0.001 < p < 0.01) or ∗ (0.01 < p < 0.05).