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Tmem88 promotes PPP specification through inhibiting Wnt/β-catenin signaling. (A) The subcellular localization of β-catenin in PPP cells of both wild-type and tmem88a/b−/− embryos in the Tg(nkx2.3:mCherry) background. Embryos were stained with the indicated antibodies. Nuclei were counterstained with DAPI (blue). The boxed area in the upper image (scale bar, 20 μm) is presented at a higher magnification in the corresponding lower image (scale bar, 100 μm). (B) Expression of nkx2.3 in wild-type or tmem88a/b−/− embryos treated with or without 20 μM CCT036477. (C) Expression of nkx2.3 in wild-type or mutant embryos injected with indicated mRNAs at 17 ss.
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