FIGURE

Fig. 2

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ZDB-FIG-241205-2

Publication

Bowley et al., 2024 - Zebrafish model for functional screening of flow-responsive genes controlling endothelial cell proliferation

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Fig. 2

Sorting of EC from fli1a:EGFP; gata1adsRed zebrafish. Approximately 40 Tg(fli1a:EGFP; gata1a:dsRed) embryos at 48 hpf were dissociated into single cells using trypsin. Using FACS, cells were gated using forward scatter (FSC) and side scatter (SSC) and then GFP + and GFP-single cells were sorted. N = 3 independent experiments were performed. (A) Typical flow cytometry data showing FSC and SSC profiles (upper panel; gating indicated as red box) and delineation of GFP + and GFP- single cells (lower panel; gating indicated as red boxes). (B) cdh5 expression was quantified in GFP + cells by qRT-PCR using actin as a control for total RNA levels. Mean 1/dCt values and individual data points are shown. Differences between means were analysed using a t-test. cdh5 expression was enriched in GFP + cells. (C) Quantification of expression of genes of interest in zebrafish endothelium. GFP + cells were analyzed by qRT-PCR. Expression of genes of interest was quantified relative to the expression of actin. Mean values + /− standard deviations and individual data points are shown. Genes which were expressed at detectable levels in one or more experiment were selected for study.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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