Figure 3

Transcriptomic analysis reveals the potential contribution of Glul in cpt1b-regulated cardiomyocyte proliferation in zebrafish. (A) Nine-quadrant plot illustrating the log2(fold change) of recovered genes in the cpt1b^−/− vs. cpt1b^+/+ analysis and cpt1b^OE vs. WT analysis. Red dots denote genes that were significantly upregulated in cpt1b^−/− hearts and concomitantly downregulated in cpt1b^OE hearts, blue dots represent genes that were downregulated in cpt1b^−/− hearts and upregulated in cpt1b^OE hearts, and pink dots highlight the two glutamine synthetase genes. (B) Gene Ontology enrichment analysis of 154 differentially expressed genes revealed significant enrichment of biological processes. The y-axis represents the enriched biological processes, and the x-axis represents the negative logarithm of the p-value. (C) Quantitative RT-PCR analysis of glula and glulb expression in cpt1b^+/+ and cpt1b^−/− hearts. (D) Quantitative RT-PCR analysis of glula and glulb expression in WT and cpt1b^OE hearts. (E) Quantitative analysis of Pcna-positive cardiomyocytes in 4 dpf WT and cpt1b^OE zebrafish embryos treated with either vehicle (Ctrl) or 250 µM MSO. n = 10–13. ** p < 0.01, *** p < 0.001, and ns indicates p > 0.05.