FIGURE

Figure 2

ID

ZDB-FIG-240729-268

Publication

Wei et al., 2024 - In Vivo Chemical Screening in Zebrafish Embryos Identified FDA-Approved Drugs That Induce Differentiation of Acute Myeloid Leukemia Cells

Other Figures

All Figure Page

Back to All Figure Page

Figure 2

Chemical screening using Tg(drl:hoxa9) identified compounds that restored myeloid differentiation. (A) A schematic representation of the chemical screening process. The effects of compounds on myeloid differentiation were examined by WISH of mpx, and Lef was used as a positive control. (B) Summary of primary and secondary screenings. (C) Tg(drl:hoxa9) embryos were treated with DMSO; Lef; or oxcarbazepine, khellin, ethacrynic acid, and alendronate and then assayed by WISH of mpx at 3 dpf. A green arrow indicates the staining signal. (D) Quantification of WISH results in (C). (E) Sibling and Tg(drl:hoxa9) embryos were treated with DMSO, Lef, oxcarbazepine, khellin, ethacrynic acid, and alendronate and stained for macrophages with NR at 5 dpf. (F) Quantification of results in (E). Results in (D,F) are represented as mean ± SEM, t-test; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p< 0.0001. NR, neutral red; Lef, leflunomide.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.