FIGURE

Fig. 3

ID

ZDB-FIG-240620-149

Publication

Marchese et al., 2024 - Targeting autophagy impairment improves the phenotype of a novel CLN8 zebrafish model

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Fig. 3

Evaluation of the pathological signs of NCL in cln8−/− mutants. (A) Immunofluorescence analysis of subunit c of mitochondrial ATP synthase (SCMAS) in 5 dpf WT and cln8−/−embryos. (B) Acridine orange staining in brains of mutant and control 24 hpf embryos. (C) Lysotracker in vivo staining in 5 dpf WT and cln8−/− larvae. (D) Bodipy staining in live 5 dpf mutant and control larvae. Three independent experiments were performed in each group: cln8−/− and controls. Statistical analysis was performed using the Mann-Whitney test (****p ≤ 0.0001; ***p ≤ 0.001; *p ≤ 0.05). (scale bar = 100 μm). The number inside the bars is the sample size. The values are expressed as mean ± standard error of the mean (SEM).

Expression Data

Antibody:	Ab1-atp5
Fish:	mitfa^w2/+; icm05Tg (AB) mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)
Anatomical Term:	trunk
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mitfa^w2/+; cln8^irc2/irc2; icm05Tg (AB)
Observed In:	brain apoptotic process brain lysosomal lumen pH elevation head lipid trunk trunk lysosomal lumen pH elevation
Stage Range:	Prim-5 to Day 5

Phenotype Detail

Acknowledgments

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