Fig. 5

YB‐1 regulates the expression of EGFR and snail. Expression levels of target gene mRNA 48 h after transfection with 5 nm of (A) si‐YB1^CDS and (B) si‐YB1^UTR, relative to control siRNA transfected cells (Co). Each dot represents the mean of one cell line, derived from three biological replicates performed in duplicates. Data are shown as mean ± SEM. Blue bars indicate a mean log2 fold change > 1. (C) Expression levels of target gene mRNA 48 h after transfection with 5 nm of si‐YB1^CDS (si‐CDS) or si‐YB1^UTR (si‐UTR) and treatment with 100 ng·mL⁻¹ doxycycline (+dox), relative to control siRNA‐transfected cells (Co). Each dot represents the mean of one cell line, derived from three biological replicates performed in duplicates. Data are shown as mean ± SEM. (D) EGFR and (E) snail protein levels relative to control‐siRNA‐transfected cells (Co) derived from densitometric analysis of western blots (N = 3), 48 h after transfection with 5 nm siRNA and treatment with or without 100 ng·mL⁻¹ doxycycline at the time of transfection as indicated. Each dot represents the mean of one cell line. Data is shown as mean ± SEM. (F) Representative pictures of the western blot.