FIGURE

Figure 10

ID

ZDB-FIG-240129-196

Publication

Sun et al., 2024 - amer1 Regulates Zebrafish Craniofacial Development by Interacting with the Wnt/β-Catenin Pathway

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Figure 10

Effects of IWR-1-endo on components of the Wnt pathway in F0 amer1 knockdown embryos. (A,B) Western blotting and quantitation showing that β-catenin expression was lower in IWR-treated amer1 knockdown embryos than in amer1 knockdown embryos at 4 dpf. (C) qPCR showing that the levels of expression of lef1, jun, and fosl1a were lower in IWR-treated than in untreated amer1 knockdown embryos. (D) Co-localization of β-catenin with the cell nucleus in the ceratohyal cartilage was reduced in amer1 knockdown embryos treated with IWR. (E) Manual counting of co-localization of β-catenin with cell nuclei in embryos with different treatments (n = 3 each). * p < 0.05; ** p < 0.01; *** p < 0.001.

Expression Data

Genes:	fosl1a jun lef1
Antibody:	Ab1-ctnnb
Fish:	TU TU + CRISPR1-amer1
Condition:	chemical treatment by environment: IWR-1-endo
Knockdown Reagent:	CRISPR1-amer1
Anatomical Terms:	chondrocyte cytoplasm chondrocyte nucleus whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + CRISPR1-amer1
Condition:	chemical treatment by environment: IWR-1-endo
Knockdown Reagent:	CRISPR1-amer1
Observed In:	chondrocyte nucleus whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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