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Recruitment of RBI-derived and CHT-derived neutrophils after tail transection. A Experimental design. At 34 h post fertilization (hpf) and 44 hpf the first and second photoconversions were performed in the head to label RBI-derived neutrophils red. At 54 hpf, caudal fin transections were made, and time lapse analysis was conducted during the 3 h post damage (hpd). B Representative images of RBI-derived/red and CHT-derived/green neutrophils at the wound site (dotted white line) at 1, 2, and 3 hpd. C Quantification of the number of RBI-derived and CHT-derived neutrophils present at the wound at 0, 1, 2, and 3 hpd. n = 16 larvae per experiment. D Representative images of RBI-derived/red and CHT-derived/green neutrophils at the wound (dotted white line) at 1, 2, and 3 hpd treated or not with diphenyliodonium (DPI). E Quantification of the number of RBI-derived and CHT-derived neutrophils that reach the wound at 1, 2, and 3 hpd treated or not with DPI. n = 10 larvae per experiment. In all the cases data are represented as mean ± SD. Statistical analysis was done with a two-sided Mann-Whitney U test. Experiments were performed independently at least 3 times. Scale bar: 100 μm.
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