FIGURE

Fig. 5

ID

ZDB-FIG-230807-5

Publication

Coltogirone et al., 2022 - Gsx2, but not Gsx1, is necessary for early forebrain patterning and long-term survival in zebrafish

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Fig. 5

Fluorescence in situ hybridization confirms minimal co-localization of gsx1 and gsx2 during late embryonic and early larval development. (Aⁱ-A^vi) Lateral views showing expression of gsx1 and gsx2 at 48 hpf. (Bⁱ-Bⁱⁱ) Ventral views showing expression of gsx1 and gsx2 at 48 hpf. (B^iv-B^vi) Cross-sectional view taken at the dashed line in A^vi. (Cⁱ-Cⁱⁱⁱ) Lateral view showing gsx1 and gsx2 expression at 72 hpf. (C^iv-C^vi) Ventral view showing gsx1 and gsx2 expression at 72 hpf. Lateral views were taken at ×20 and ventral/Cross-sectional views were taken at ×40, all with anterior facing left. All were pseudocolored using FIJI ImageJ and scale bars represent 100 μm. For lateral views, eyes were dissected off. Main images are max z-projections and insets are single z-stack slices zoomed into the boxed region shown in the main image. CeP, cerebellar plate; Di, diencephalon; Hb, hindbrain; Hyp, hypothalamus; Mb, midbrain; OB, olfactory bulb; P, pallium; Po, preoptic area; Pr, pretectum; Sc, spinal cord; Tel, telencephalon; TeO, optic tectum

Expression Data

Genes:	gsx1 gsx2
Fish:	WT
Anatomical Terms:	dorsal telencephalon hypothalamus olfactory bulb optic tectum preoptic area pretectum (all 6)
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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