FIGURE

Fig 10

ID

ZDB-FIG-220319-59

Publication

Liu et al., 2022 - Rod genesis driven by mafba in an nrl knockout zebrafish model with altered photoreceptor composition and progressive retinal degeneration

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Fig 10

Progressive degeneration and regeneration in the <italic toggle='yes'>nrl</italic>-KO retinas.

(A) The TUNEL assay results revealed that multiple types of retinal cells, including rods, cones, RPE, and inner retinal cells, undergo apoptosis in the nrl-KO retinas. Representative images are shown. Scale bar: 20 μm. (B) Quantitation of the apoptotic cells per section from 5 mpf to 13 mpf. The results are shown as mean with SD (n = 3). *, p < 0.05; **, p < 0.01. (C) RPE morphology in the WT and nrl-KO zebrafish at 20 mpf are shown, as assessed by immunostaining the retinal whole-mounts for ZO-1 Scale bar: 20 μm. (D) The up-regulation of GFAP in nrl-KO retinas as detected by immunostaining. CN, cone nuclear layer; RN, rod nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar: 25 μm. (E) Regeneration of photoreceptors was more active in the nrl-KO retinas at 9 mpf and 18 mpf, as reflected by the increase in the number of Pcna-positive cells (proliferating cells) in the ONL, compared with the WT levels. Scale bars: 50 μm. (F) Quantitation of the Pcna⁺ cells located in the ONL per section. The results are shown as mean with SD (n = 4) from 7 dpf to 29 mpf. *, p < 0.05; **, p < 0.01.

Expression Data

Antibodies:	Ab7-tjp1 Ab17-pcna Ab22-gfap
Fish:	WT nrl^hzu16/hzu16
Anatomical Terms:	retinal ganglion cell layer retinal photoreceptor layer retinal pigmented epithelium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nrl^hzu16/hzu16
Observed In:	retinal cone cell apoptotic process retinal ganglion cell layer retinal inner nuclear layer apoptotic process retinal photoreceptor layer retinal photoreceptor layer cell population proliferation retinal pigmented epithelium apoptotic process retinal rod cell apoptotic process (all 7)
Stage Range:	Days 7-13 to Adult

Phenotype Detail

Acknowledgments

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