FIGURE

Fig 2

ID

ZDB-FIG-191230-1145

Publication

Watterston et al., 2019 - MicroRNA26 attenuates vascular smooth muscle maturation via endothelial BMP signalling

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Fig 2

<italic>miR26a</italic> knockdown increases <italic>smad1</italic> expression.

A) Schematic of miR26a transient knockdown methods. B and C) Relative expression level of miR26a in morpholino and CRISPRi injected embryos at 48 hpf (n = 3). D) Whole mount in situ hybridization staining for smad1 at 48 hpf and 4 dpf shows increased expression of smad1 in miR26a knockdown embryos particularly in the ventral aorta, aortic arches and pharyngeal region (dotted outline). Scale bar represents 200μm. E) Relative expression of smad1 in 48 hpf morphants is increased compared to control embryos (n = 3). F) Relative expression of smad1 in 4 dpf miR26a morphants is increased compared to control embryos (n = 4). RT-qPCR data show the mean ± SEM, Student's two-tailed t-test *p < 0.05, n = number of biological replicates.

Expression Data

Gene:	smad1
Fish:	WT WT + CRISPR1-mir26a WT + MO1-mir26a
Knockdown Reagents:	CRISPR1-mir26a MO1-mir26a
Anatomical Terms:	aortic arch head pharyngeal arch pharyngeal arch ventral region ventral aorta whole organism (all 6)
Stage Range:	Long-pec to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-mir26a WT + MO1-mir26a
Knockdown Reagents:	CRISPR1-mir26a MO1-mir26a
Observed In:	aortic arch head pharyngeal arch ventral region ventral aorta whole organism
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

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