FIGURE

Fig 5

ID

ZDB-FIG-190723-796

Publication

Song et al., 2019 - HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development

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Fig 5

Hdac1 is required for the proliferation of SHF progenitors.

(A-B”‘) Confocal images of IHC for hearts and Nkx2.5⁺ SHF progenitors in WT sibling and crg mutant embryos at 33 hpf. Nkx2.5⁺ (green), PHH3 (blue) and MHC (purple). Outline in A and B indicates Nkx2.5⁺/MHC^- SHF. Arrows indicate Nkx2.5⁺/MHC^-/PHH3⁺ cells. Yellow arrow indicates Nkx2.5⁺/MHC^-/pHH3⁺ cell of the higher magnification inset. (A”“and B”“) Schematic indicating IHC from A and B. Green indicates Nkx2.5⁺/MHC^- cells. Blue indicates Nkx2.5⁺/MHC^-/PHH3⁺ cells. Purple indicates MHC⁺ cells. Anterior is up in A-B”“. (C) Quantification of SHF progenitors (Nkx2.5⁺/MHC^-). (D) Percentage of PHH3⁺ SHF progenitors. For C and D, n = 14 for WT and n = 15 crg mutants. (E) RT-qPCR for cdkn1a from sorted nkx2.5:ZsYellow⁺ cells.

Expression Data

Gene:	cdkn1a
Antibodies:	Ab-MF20 Ab3-nkx2.5 Ab7-h3
Fish:	WT hdac1^nl18/nl18 fb7Tg + MO1-hdac1 + MO4-tp53
Knockdown Reagents:	MO1-hdac1 MO4-tp53
Anatomical Terms:	cardiac muscle cell heart tube presumptive bulbus arteriosus
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hdac1^nl18/nl18 fb7Tg + MO1-hdac1 + MO4-tp53
Knockdown Reagents:	MO1-hdac1 MO4-tp53
Observed In:	bulbus arteriosus cardiac muscle cell presumptive bulbus arteriosus presumptive bulbus arteriosus cardiac muscle cell proliferation
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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