FIGURE

Fig 4

ID

ZDB-FIG-190723-1595

Publication

Chen et al., 2019 - Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition

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Fig 4

Perturbation of <italic>klf4</italic> expression affects the densities of NaR and HR cells at 24 hpf.

(A) The density of atp1a1a.1⁺-Na⁺-K⁺-ATPase-rich (NaR) cells was reduced in yolk extensions of embryos injected with both klf4 MO1 and MO2 (c) as compared to uninjected wild types (a) and control embryos injected with klf4 5mmMO2 (b). The density of atp1a1a.1⁺-NaR cells was increased in yolk extensions of embryos injected with klf4 (g) mRNA, as compared to embryos injected with the same amount of LacZ (f) mRNA, or uninjected wild type (e) embryos. Quantification of results from (a-c) and (e-g) are shown in (d) and (h), respectively. (B) The density of atp6v1aa⁺- H⁺-ATPase-rich (HR) cells was reduced in yolk extensions of embryos injected with both klf4 MO1 and MO2 (c) as compared to uninjected wild types (a) and control embryos injected with klf4 5mmMO2 (b). The density of atp6v1aa⁺-HR cells was increased in yolk extensions of embryos injected with klf4 (g) mRNA, as compared to embryos injected with the same amount of LacZ (f) mRNA, or uninjected wild-type (e) embryos. Quantification of results from (a-c) and (e-g) are shown in (d) and (h), respectively. Statistical significance was determined by Student’s t-test. ***p < 0.001. Scale bar, 300 μm. Error bars indicate standard error.

Expression Data

Genes:	atp1a1a.1 atp6v1aa
Fish:	WT WT + MO1-klf4 + MO2-klf4 WT + MO2-klf4
Knockdown Reagents:	MO1-klf4 MO2-klf4
Anatomical Terms:	extension NaK ionocyte extension vH ionocyte
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-klf4 + MO2-klf4
Knockdown Reagents:	MO1-klf4 MO2-klf4
Observed In:	extension NaK ionocyte extension vH ionocyte
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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