FIGURE

Fig. 3

ID

ZDB-FIG-180806-7

Publication

Rothschild et al., 2018 - Calcium Signals Act Through Histone Deacetylase to Mediate Pronephric Kidney Morphogenesis

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Fig. 3

HDACs have opposing effects on primary cilia. A–D: Lateral views of 72‐hpf embryos immunostained for acetylated tubulin (green) reveal cilia at the junction of a pronephric duct with the cloaca. Scale bar = 5 μm. Cloacal cilia number and length were assessed at 72 hpf (E,F). n = 15–30 embryos. *P < 0.005 in comparison to controls. Cardiac asymmetry was analyzed by cmlc2 expression at 24 hpf (G–I) and plotted as a percentage of embryos (J). n = 20–38 embryos. Primary cilia length in the KV at the 12‐somite stage (K–M) is averaged (N). n = 40–60 cilia. Scale bar = 5 μm. *P < 0.005.

Expression Data

Antibody:	Ab1-tuba
Fish:	WT WT + MO4-hdac5 WT + MO5-hdac6 WT + MO6-hdac4
Knockdown Reagents:	MO4-hdac5 MO5-hdac6 MO6-hdac4
Anatomical Term:	cloaca cilium
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO4-hdac5 WT + MO6-hdac4
Knockdown Reagents:	MO4-hdac5 MO6-hdac4
Observed In:	cloaca cilium determination of heart left/right asymmetry Kupffer's vesicle cilium
Stage Range:	5-9 somites to Protruding-mouth

Phenotype Detail

Acknowledgments

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