FIGURE

Fig. 1

ID
ZDB-FIG-180221-27
Publication
Jou et al., 2017 - A Functional Assay for Sick Sinus Syndrome Genetic Variants
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Fig. 1

Embryonic hnc4 expression: whole-mount in situ hybridization reveals hcn4 expression in the heart (arrow) at (A) 18 somites, (B) 24 hpf and (C) 48 hpf. To explore the mechanism by which hcn4 functions in zebrafish, we bathed whole embryos in 4-(N-ethyl-N-phenylamino)-1,2-dimethyl-6-(methylamino) pyrimidinium chloride (ZD-7288), a nonselective hcn blocker, and assayed the heart rate at 48 hpf. We found that embryos bathed in 1 mM ZD-7288 had significantly slower heart rates than untreated embryos (Fig. 2A). However, cardiac pauses were not observed in embryos exposed to ZD-7288.

Expression Data
Gene:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Long-pec

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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