FIGURE

Fig. 5

ID

ZDB-FIG-171002-5

Publication

Shimizu et al., 2017 - The Calcineurin-FoxO-MuRF1 signaling pathway regulates myofibril integrity in cardiomyocytes

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Fig. 5

Blocking MuRF1-mediated proteasome degradation preserves myofibril integrity in tre/ncx1 deficient hearts.

(A) Z-lines were visualized by α-actinin staining. By 72 hpf, sarcomeres are disassembled in hearts of uninjected (tre) and control morpholino-injected (tre +ctlMO) tremblor embryos. Murf1a/murf1 b knockdown does not affect sarcomere integrity in wild type embryos (WT +MO), but prevents sarcomere degradation in tre (tre +MO). Similarly, treatment with a proteasome inhibitor, MG132, preserves myofibril integrity in tre cardiomyocytes (tre +MG132). Scale bar, 10 μm. (B) Graph shows % of embryos with periodic α-actinin staining at 72 hpf. (C) Western blot detecting MuRF1 and β-actin proteins in uninjected control (WT control) and murf1a/murf1 b knockdown (MuRF1 MO) embryos. Chi-squared test, *p<0.05; **p<0.01; ***p<0.001.

Expression Data

Antibody:	Ab1-trim63
Fish:	WT WT + MO1-trim63a + MO1-trim63b
Knockdown Reagents:	MO1-trim63a MO1-trim63b
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc8a1a^{tc318d/tc318d} WT + MO1-trim63a + MO1-trim63b slc8a1a^{tc318d/tc318d} + MO1-trim63a + MO1-trim63b
Condition:	chemical treatment: proteasome inhibitor
Knockdown Reagents:	MO1-trim63a MO1-trim63b
Observed In:	cardiac muscle cell sarcomere cardiac muscle cell Z disc whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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