FIGURE

Fig. S6

ID
ZDB-FIG-170914-43
Publication
Nguyen-Chi et al., 2017 - TNF signaling and macrophages govern fin regeneration in zebrafish larvae
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Fig. S6

Effect of tnfa morpholino on caudal fin regeneration (a) Fin images are representative transmitted light images of Ctrl morphants and tnfa morphants at 3dpA. (b) Corresponding regenerated fin length (mean ±s.e.m. from three independent experiments, ***P<0.001). (c) Schematic representation of the parabiosis experiment using tnfa MO or WT (CTRL) and Tg(mpeg1:GAL4/UAS:NTR-mCherry). Parabiotic larvae were treated with MTZ at 48 hpf and the caudal fin of Tg(mpeg1:GAL4/UAS:NTR-mCherry) was amputated at 3 dpf. (d) Representative fin images of mCherry fluorescence merged with transmitted channel at 6 hpA show NTRmCherry+ macrophages that are mainly depleted. White arrowheads show residual fluorescence in cells or cell fragments. (e) Fin images are representative transmitted light images at 3 dpA in tnfa morphants and CTRL. (f) Corresponding quantification of the regenerated fin length in indicated conditions (Nlarvae=8-10 mean ± s.e.m., *P<0.05). In (a, d and e) dotted lines outline the fin, dashed arrows indicate the position of the initial transection. Scale bars = 100 μm.
Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.
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