FIGURE

Fig. 6

ID

ZDB-FIG-160708-7

Publication

He et al., 2016 - Synergy between loss of NF1 and overexpression of MYCN in neuroblastoma is mediated by the GAP-related domain

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Fig. 6

Loss of nf1 results in aberrant ERK/Akt/mTOR signaling in MYCN-driven neuroblastoma.

Immunohistochemical analysis of sagittal sections through tumors in the IRG of nf1a+/+;nf1b+/+;MYCN;GFP (A,D,G), nf1a+/-;nf1b+/+;MYCN;GFP (B,E,H) and nf1a-/-;nf1b+/+;MYCN;GFP (C,F,I) fish at the age of 6 weeks, using antibodies against phosphorylated ERK1/2 (pERK, A-C), phosphorylated AKT (pAKT, D-E) and phosphorylated S6 (pS6, G- I). Dotted lines indicate the tumor boundaries. The quantification of pERK-, pAKT- and pS6-positive tumor areas are shown in (J), with the red bars representing the median values. ns p>0.05, *p<0.05, **p<0.01 by two-tailed unpaired t-test.

Expression Data

Antibodies:	Ab5-akt Ab9-mapk Ab10-rps6
Fish:	zdf16Tg nf1a^p301/+; zdf16Tg nf1a^p301/p301; zdf16Tg
Anatomical Term:	interrenal gland neuroblastoma
Stage:	Days 30-44

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zdf16Tg nf1a^p301/+; zdf16Tg nf1a^p301/p301; zdf16Tg
Observed In:	interrenal gland neuroblastoma
Stage:	Days 30-44

Phenotype Detail

Acknowledgments

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