Fig. 1
- ID
- ZDB-FIG-151203-11
- Publication
- Voelz et al., 2015 - A zebrafish larval model reveals early tissue-specific innate immune responses to Mucor circinelloides
- Other Figures
- (all 7)
- All Figure Page
- Back to All Figure Page
Zebrafish larvae are susceptible to the mucormycete M. circinelloides NRRL3631 in a hindbrain infection model. (A) AB wild-type zebrafish larva were injected in the hindbrain ventricle at prim-25 stage with a low dose (LD Mc, approximately 10 spores) or a high dose of Mucor circinelloides NRRL3631 (HD Mc, approximately 100 spores) and monitored over time (representative z-stacks: 28, 27, 23 and 40 sections every 3µm, respectively; scale bar: 40µm). (B) Injection of LD and HD Mc mucormycete spores into the hindbrain ventricle of prim-25 larvae induced significant mortality. Hindbrain ventricle infection with UV-killed spores did not cause significant mortality in AB wild-type larvae compared with the PVP control. (C) Hyphal growth invading the forebrain and ventral muscular layers from posterior hindbrain (representative images of HD Mc infection; top and middle right: DIC montage with filamentous growth highlighted in blue; fluorescent z-stacks: 61 sections every 4.4µm; scale bar: 50µm, upper two panels). Dead fish, previously injected with viable spores, presented with filamentous fungal growth (representative image from LD Mc hindbrain injection; cyan, spores/hyphae; red, macrophages; scale bar: 100µm). (D) Spores remained viable within the zebrafish larvae over the time course of 48h post-infection (h.p.i.). (E,F) Larval immunosuppression increases susceptibility to infections with M. circinelloides NRRL3631. (E) AB wild-type zebrafish larvae treated with dexamethasone after hindbrain ventricle injection showed significantly increased mortality at the low as well as the high infection dose compared with DMSO-treated infected larvae. (F) In comparison to untreated infected Tg(mpeg1:G/U:NfsB-mCherry) larvae, larval treatment with metronidazole (Met) significantly increased susceptibility to low- and high-dose hindbrain ventricle infection with fungal spores. |
Gene: | |
---|---|
Fish: | |
Condition: | |
Anatomical Term: | |
Stage: | Pec-fin |
Fish: | |
---|---|
Conditions: | |
Observed In: | |
Stage Range: | Prim-25 to Days 7-13 |